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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
29/12/2015 |
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Data da última atualização: |
03/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, K. C. de; ANDRIOLI, A.; SIDER, L. H.; PINHEIRO, R. R.; BEZERRA JUNIOR, R. Q.; PEIXOTO, R. M.; TEIXEIRA, M. F. da S. |
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Afiliação: |
Kelma Costa de Souza, Pós-graduação - Universidade Estadual do Ceará (UECE) - Fortaleza, CE; ALICE ANDRIOLI, CNPC; LUCIA HELENA SIDER, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; Rosivaldo Quirino Bezerra Junior; Renato Mesquita Peixoto; Maria Fátima da Silva Teixeira. |
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Título: |
Detecção de sequências do DNA proviral do vírus da Artrite Encefalite Caprina em saliva. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Acta Scientiae Veterinariae, v. 43, n. 1266, p. 1-6, 2015. |
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Idioma: |
Português |
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Conteúdo: |
[Detection of Proviral Sequences of the Caprine Arthritis Encephalitis Virus in Saliva]. Background: Goats can be infected by a lentivirus called the caprine arthritis encephalitis virus (CAEV) that causes an infectious disease characterized by a chronic. For replication, the CAEV, integrate as a provirus in the DNA of the host cell genome. By consequence, infection of cells persistent life-long infection of the animal. The CAEV can be found in most body fluids, It has been demonstrated its presence in blood, milk, semen. However, the search for the CAEV in other body fluids besides blood is important to assess possible viral transmission. The aim of this study was to determine whether the presence of proviral DNA sequences in saliva of animals infected. Materials, Methods & Results: The study was carried out on the farm belonging to the Embrapa Goat and Sheep Research Center, located in the municipality of Sobral, Ceará, Brazil. To assess the oral fluid for the presence of CAEV, samples of saliva from eight infected breeders were collected by suctioning saliva from the oral cavity on the side region of the breeders lower molar teeth using a probe coupled to a plastic 5 mL syringe. And pro-viral DNA was extracted from the samples using NaCl and proteinase K. Two rounds of polymerase chain reaction (nested PCR) were carried out to amplify the final 187 pb fragment of the pro-viral DNA. All the oligonucleotide primers were determined from the gag region of the structural gene of the CAEV-Cork. The fragments amplified by PCR were analyzed in electrophoresis in a horizontal cube. On 2% agarose gel and stained with ethidium bromide (10?g/?L). The products of the PCR ampli?cation were sequenced in platform Applied Biosystems, the sequences were compared to available GenBank strains, and the results of this comparison demonstrated that were more related to the caprine strains regional. The presence of pro-viral DNA was observed in saliva in of 100% animals and proved that saliva of infected goats contained the CAEV. However, when assessing the results of CAEV pro-viral DNA detection in the present study, it was observed variation in the detection frequency of lentivirus in samples collected at different times. Discussion: This finding indicated that the CAEV was present in saliva samples in its pro-viral form, a factor that would not limit its contagion capacity and that direct, continued exposure in a favorable environment presents perfect conditions for its transmission by this pathway. The results found in the present study corroborated studies on other lentivirus. Isolated the maedi-visna virus (MVV) in saliva glands of experimentally infected sheep. In cats, the feline immunodeficiency virus (FIV) was also detected in the saliva of infected animals and transmission was reported. In a recent study, was detected the presence of the lentivirus in small ruminants by PCR in water and air samples in different installations that housed herds with medium to high infection prevalence. Throughout in this study, an intermitency in proviral elimination was observed in saliva similar blood and semen, as reported by other authors for CAEV and other virus. Although the determining mechanism of horizontal transmission through the oral fluids was not definitively elucidated, these studies highlight the risks of transmission through direct contact with animals or indirect contamination by food and/or water in the drinker. This study proved that saliva from infected goats contains the CAEV, more studies should be carried out to analyze the degree of infectiousness of the virus in the saliva and the viral elimination characteristics in this medium. Menos[Detection of Proviral Sequences of the Caprine Arthritis Encephalitis Virus in Saliva]. Background: Goats can be infected by a lentivirus called the caprine arthritis encephalitis virus (CAEV) that causes an infectious disease characterized by a chronic. For replication, the CAEV, integrate as a provirus in the DNA of the host cell genome. By consequence, infection of cells persistent life-long infection of the animal. The CAEV can be found in most body fluids, It has been demonstrated its presence in blood, milk, semen. However, the search for the CAEV in other body fluids besides blood is important to assess possible viral transmission. The aim of this study was to determine whether the presence of proviral DNA sequences in saliva of animals infected. Materials, Methods & Results: The study was carried out on the farm belonging to the Embrapa Goat and Sheep Research Center, located in the municipality of Sobral, Ceará, Brazil. To assess the oral fluid for the presence of CAEV, samples of saliva from eight infected breeders were collected by suctioning saliva from the oral cavity on the side region of the breeders lower molar teeth using a probe coupled to a plastic 5 mL syringe. And pro-viral DNA was extracted from the samples using NaCl and proteinase K. Two rounds of polymerase chain reaction (nested PCR) were carried out to amplify the final 187 pb fragment of the pro-viral DNA. All the oligonucleotide primers were determined from the gag region of the structural gene ... Mostrar Tudo |
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Palavras-Chave: |
Artrite-encefalite Caprina; CAEV; Caprine arthritis encephalit virus; DNA sequence; PCR. |
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Thesagro: |
Caprino; Doença animal; Saliva; Transmissão de doença; Vírus. |
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Thesaurus Nal: |
Animal diseases; Disease transmission; Goats. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/136302/1/cnpc-2015-Deteccao.pdf
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Marc: |
LEADER 04687naa a2200349 a 4500
001 2032574
005 2019-04-03
008 2015 bl uuuu u00u1 u #d
100 1 $aSOUZA, K. C. de
245 $aDetecção de sequências do DNA proviral do vírus da Artrite Encefalite Caprina em saliva.$h[electronic resource]
260 $c2015
520 $a[Detection of Proviral Sequences of the Caprine Arthritis Encephalitis Virus in Saliva]. Background: Goats can be infected by a lentivirus called the caprine arthritis encephalitis virus (CAEV) that causes an infectious disease characterized by a chronic. For replication, the CAEV, integrate as a provirus in the DNA of the host cell genome. By consequence, infection of cells persistent life-long infection of the animal. The CAEV can be found in most body fluids, It has been demonstrated its presence in blood, milk, semen. However, the search for the CAEV in other body fluids besides blood is important to assess possible viral transmission. The aim of this study was to determine whether the presence of proviral DNA sequences in saliva of animals infected. Materials, Methods & Results: The study was carried out on the farm belonging to the Embrapa Goat and Sheep Research Center, located in the municipality of Sobral, Ceará, Brazil. To assess the oral fluid for the presence of CAEV, samples of saliva from eight infected breeders were collected by suctioning saliva from the oral cavity on the side region of the breeders lower molar teeth using a probe coupled to a plastic 5 mL syringe. And pro-viral DNA was extracted from the samples using NaCl and proteinase K. Two rounds of polymerase chain reaction (nested PCR) were carried out to amplify the final 187 pb fragment of the pro-viral DNA. All the oligonucleotide primers were determined from the gag region of the structural gene of the CAEV-Cork. The fragments amplified by PCR were analyzed in electrophoresis in a horizontal cube. On 2% agarose gel and stained with ethidium bromide (10?g/?L). The products of the PCR ampli?cation were sequenced in platform Applied Biosystems, the sequences were compared to available GenBank strains, and the results of this comparison demonstrated that were more related to the caprine strains regional. The presence of pro-viral DNA was observed in saliva in of 100% animals and proved that saliva of infected goats contained the CAEV. However, when assessing the results of CAEV pro-viral DNA detection in the present study, it was observed variation in the detection frequency of lentivirus in samples collected at different times. Discussion: This finding indicated that the CAEV was present in saliva samples in its pro-viral form, a factor that would not limit its contagion capacity and that direct, continued exposure in a favorable environment presents perfect conditions for its transmission by this pathway. The results found in the present study corroborated studies on other lentivirus. Isolated the maedi-visna virus (MVV) in saliva glands of experimentally infected sheep. In cats, the feline immunodeficiency virus (FIV) was also detected in the saliva of infected animals and transmission was reported. In a recent study, was detected the presence of the lentivirus in small ruminants by PCR in water and air samples in different installations that housed herds with medium to high infection prevalence. Throughout in this study, an intermitency in proviral elimination was observed in saliva similar blood and semen, as reported by other authors for CAEV and other virus. Although the determining mechanism of horizontal transmission through the oral fluids was not definitively elucidated, these studies highlight the risks of transmission through direct contact with animals or indirect contamination by food and/or water in the drinker. This study proved that saliva from infected goats contains the CAEV, more studies should be carried out to analyze the degree of infectiousness of the virus in the saliva and the viral elimination characteristics in this medium.
650 $aAnimal diseases
650 $aDisease transmission
650 $aGoats
650 $aCaprino
650 $aDoença animal
650 $aSaliva
650 $aTransmissão de doença
650 $aVírus
653 $aArtrite-encefalite Caprina
653 $aCAEV
653 $aCaprine arthritis encephalit virus
653 $aDNA sequence
653 $aPCR
700 1 $aANDRIOLI, A.
700 1 $aSIDER, L. H.
700 1 $aPINHEIRO, R. R.
700 1 $aBEZERRA JUNIOR, R. Q.
700 1 $aPEIXOTO, R. M.
700 1 $aTEIXEIRA, M. F. da S.
773 $tActa Scientiae Veterinariae$gv. 43, n. 1266, p. 1-6, 2015.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 37 | |
| 5. |  | SOUZA, K. C. de; ANDRIOLI, A.; SANTOS, D. O. Efeitos de diferentes liberadores de progesterona utilizados num programa de sincronização de estro para inseminação (IATF) em cabras SRD. In: CONGRESSO BRASILEIRO DE ZOOTECNIA, 19.; CONGRESSO INTERNACIONAL DE ZOOTECNIA, 11; FÓRUM DE COORDENADORES DE CURSOS DE ZOOTECNIA DAS UNIVERSIDADES BRASILEIRAS, 5.; FÓRUM DE ESTUDANTES DE CURSOS DE ZOOTECNIA DAS UNIVERSIDADES BRASILEIRAS, 5.; REUNIÃO NACIONAL DE ENSINO DE ZOOTECNIA, 15.; FÓRUM DE ENTIDADES DE ZOOTECNISTAS, 22. 2009, Águas de Lindóia. Visão estratégica de cadeias do agronegócio: anais. Pirassununga: Faculdade de Zootecnia e Engenharia de Alimentos: Associação Brasileira de Zootecnistas, 2009. Zootec 2009. 3 f. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
|   |
| 8. | | ANDRIOLI, A.; PINHEIRO, R. R.; SOUZA, K. C. de; SOUSA, F. M. L. Avaliação da possibilidade de transmissão do vírus da artrite encefalite caprina (CAEV) pela monta natural controlada. In: CONGRESSO BRASILEIRO DE MEDICINA VETERINÁRIA, 36.; ENCONTRO BRASILEIRO DE MÉDICOS VETERINÁRIOS DAS FORÇAS ARMADAS, 1.; ENCONTRO DO COLÉGIO BRASILEIRO DE AQUICULTURA, 6.; ENCONTRO DE MEDICINA DE ANIMAIS AQUÁTICOS, 1.; ENCONTRO DOS MÉDICOS VETERINÁRIOS DA BAHIA, 68., 2009, Porto Seguro. Inovação e responsabilidade social: anais. Porto Seguro: SBMV, 2009. 3 f. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
|  |
| 12. | | ANDRIOLI, A.; PINHEIRO, R. R.; SOUZA, K. C. de; SANTOS, D. O.; SOUSA, F. M. L. Estudo sobre a transmissão do Vírus da Artrite Encefalite Caprina (CAEV) através da inseminação artificial utilizando sêmen de animal infectado. In: SIMPÓSIO INTERNACIONAL SOBRE CAPRINOS E OVINOS DE CORTE, 4.; FEIRA NACIONAL DO AGRONEGÓCIO DA CAPRINO-OVINOCULTURA DE CORTE, 3., 2009, João Pessoa. Anais... João Pessoa: EMEPA-PB, 2009. 3 f. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 13. | | SOUZA, K. C. de; ANDRIOLI, A.; PINHEIRO, R. R.; BRITO, R. L. L. de; RODRIGU, A. de S. Métodos indiretos de diagnóstico do lentivírus caprino. In: CONGRESSO BRASILEIRO DE ZOOTECNIA, 19.; CONGRESSO INTERNACIONAL DE ZOOTECNIA, 11; FÓRUM DE COORDENADORES DE CURSOS DE ZOOTECNIA DAS UNIVERSIDADES BRASILEIRAS, 5.; FÓRUM DE ESTUDANTES DE CURSOS DE ZOOTECNIA DAS UNIVERSIDADES BRASILEIRAS, 5.; REUNIÃO NACIONAL DE ENSINO DE ZOOTECNIA, 15.; FÓRUM DE ENTIDADES DE ZOOTECNISTAS, 22. 2009, Águas de Lindóia. Visão estratégica de cadeias do agronegócio: anais. Pirassununga: Faculdade de Zootecnia e Engenharia de Alimentos: Associação Brasileira de Zootecnistas, 2009. Zootec 2009. 3 p. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 14. | | AZEVEDO, D. A. A.; ALVES, S. M.; SOUZA, K. C. de; SOUSA, T. B. C.; ANDRIOLI, A.; PINHEIRO, R. R. Isolamento do Lentivírus Caprino pela técnica de cultivo de macrófagos e confirmação por PCR Nested. In: SIMPÓSIO INTERNACIONAL SOBRE CAPRINOS E OVINOS DE CORTE, 5.; FEIRA NACIONAL DO AGRONEGÓCIO DA CAPRINO-OVINOCULTURA DE CORTE, 3., 2011, João Pessoa. [Anais...]. João Pessoa: [SEBRAE-PB]; EMEPA-PB, 2011. 3 f. 1 CD ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 15. | | ABREU, D. A. de; ANDRIOLI, A.; BRITO, R. L. L. de; ÁVILA, A. A.; PINHEIRO, R. R.; SOUZA, K. C. de. Avaliação da massa corporal e do índice articular clínico em caprinos portadores do vírus da Artrite-Encefalite Caprina (CAEV). In: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 6.; SIMPÓSIO NORDESTINO DE ALIMENTAÇÃO DE RUMINANTES, 7.; FÓRUM DE COORDENADORES DE PÓS GRADUAÇÃO EM PRODUÇÃO ANIMAL DO NORDESTE, 1.; FÓRUM DE AGROECOLOGIA RO RIO GRANDE DO NORTE, 1., 2010, Mossoró. Anais... Mossoró: Sociedade Nordestina de Producao Animal; UFERSA, 2010. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 16. | | VERAS, A. K. A.; BRITO, R. L. L. de; RODRIGUES, A. de S.; SOUZA, K. C. de; OLIVEIRA, E. L.; ANDRIOLI, A.; SIDER, L. H.; PINHEIRO, R. R. Avaliação do RT-nested PCR como método de diagnóstico da artrite-encefalite caprina em cabritos com sintomatologia nervosa. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA UNIVERSIDADE ESTADUAL VALE DO ACARAÚ, 7., 2010, Sobral. Iniciação para o semiárido: [Resumos]. Sobral: Universidade Estadual Vale do Acaraú, 2010. 1 CD ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 17. | | SOUSA, S. D.; SILVA, P. A. F. da; SOUZA, K. C. de; VERAS, A. K. A.; LOPES, T. de A.; ANDRIOLI, A. Avaliação de técnicas biomoleculares em amostras de sangue e ovócitos de fêmeas infectadas com vírus da Artrite Encefalite Caprina. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA UNIVERSIDADE ESTADUAL VALE DO ACARAÚ, 13., 2011, Sobral. [Resumos...]. Sobral: Universidade Estadual Vale do Acaraú, 2011.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 18. | | SIDER, L. H.; BRITO, R. L. L. de; VERAS, A. K. A.; RODRIGUES, A. S.; SOUZA, K. C. de; OLIVEIRA, E. L. de; ANDRIOLI, A.; PINHEIRO, R. R. Processamento de liquido cefalo-raquidiano para extração de RNA genômico do vírus da artrite-encefalite caprina e diagnóstico molecular por RT-nested PCR. Sobral: Embrapa Caprinos e Ovinos, 2010. 6 p. (Embrapa Caprinos e Ovinos. Comunicado Técnico, 114). Metodologia Científica.| Tipo: Comunicado Técnico/Recomendações Técnicas |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 19. | | PAULA, N. R. de O.; ANDRIOLI, A.; CARDOSO, J. de F. S.; PINHEIRO, R. R.; SOUZA, K. C. de; ALVES, F. S. F. Perfil clínico e andrológico de bodes Moxotó e Canindé infectados experimentalmente com lentivírus de pequenos ruminantes (LVPR). Ciência Animal, Fortaleza, v. 22, n. 1, p. 457-459, 2012. suplemento. Trabalho apresentado no 6o. Congresso Norte Nordeste de Reprodução animal, 2012, Fortaleza.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 20. | | SOUZA, K. C. de; PINHEIRO, R. R.; SIDER, L. H.; BRITO, R. L. L. de; RODRIGUES, A. de S.; ANDRIOLI, A. Infecção experimental em cabras pelo vírus da artrite encefalite caprina através da inseminação artificial. In: CONGRESSO BRASILEIRO DE MEDICINA VETERINÁRIA, 36.; ENCONTRO BRASILEIRO DE MÉDICOS VETERINÁRIOS DAS FORÇAS ARMADAS, 1.; ENCONTRO DO COLÉGIO BRASILEIRO DE AQUICULTURA, 6.; ENCONTRO DE MEDICINA DE ANIMAIS AQUÁTICOS, 1.; ENCONTRO DOS MÉDICOS VETERINÁRIOS DA BAHIA, 68., 2009, Porto Seguro. Inovação e responsabilidade social: anais. Porto Seguro: SBMV, 2009. 3 f. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 37 | |
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